Our Single Primer Extension Service is offered for Plasmids, PCR Products, Single-Strand templates like M13 clones, and larger constructs such as BAC clones. The results from our Single Primer Extension Service are generally available within 18 hours of submission.
If you are looking to sequence PCR products, Sequetech offers a PCR Product Purification service. Our PCR Product Purification service is optimized to dovetail with our sequencing protocol and allows data to continue to be available the next morning without a delay.
There are multiple ways to submit samples:
Here is what is included in this service:
Template Type | Amount | Concentration | Notes |
---|---|---|---|
Plasmids | 200ng/Primer Extension | >50ng/ul | Submitting extra will allow for re-runs, optimization and troubleshooting. Primers and templates are stored for at least 3 months. We strongly recommend confirming PCR products by gel analysis. |
PCR (>800bp) | 50ng/Primer Extension | >10ng/ul | |
PCR(<800bp) | 10ng/Primer Extension | >2ng/ul |
Amount | Minimum Concentration |
Notes |
---|---|---|
4 pmole/primer extension | 2 uM | Submitting extra will allow for optimization and troubleshooting. Primers and templates are stored for at least 3 months. |
Click here to place an order or contact us if you have any questions about the service or to obtain pricing for your account!
Our Singler Primer Extension can also be performed on intact (non-prepped) cells. Please visit our Rolling Circle Amplification page for more details.
Have any questions about the service or would like pricing? Please email us at info@sequetech.com or call 650-967-8685.