Container:
For overnight shipping, cap strips are highly recommended. Adhesive sheets or foil sometimes leak. Clearly label all plates.
Temperature:
Dry Ice or Room Temperature. Please do not use wet ice.
Container:
Tubes should have a tight fitting cap. Clearly label all tubes. Please do not send PCR cap strip tubes.
Temperature:
Dry Ice or Room Temperature. Please do not use wet ice.
Container:
Liquid Cultures or Glycerol Stocks for RCA should be shipped with tight fitting caps for samples sent in tubes. For plates, cap strips are highly recommended. Adhesive sheets or foil sometimes leak. Clearly label all plates. We recommend that you wrap your shipment with bubble wrap to protect the contents.
Temperature:
Please ship at ROOM TEMPERATURE. Please do not use any type of ice.
Container:
If picked into tubes, the tubes should have a tight fitting cap. If picked into plates, cap strips are highly recommended. Adhesive sheets or foil sometimes leak. We recommend that you wrap your shipment with bubble wrap to protect the contents. Please do not send PCR cap strip tubes.
Temperature:
Please ship at ROOM TEMPERATURE. Please do not use any type of ice.
Container:
Agar plates can be shipped as is. They must be wrapped in parafilm. We recommend that you also pad your agar plate with bubble wrap when shipping.
Temperature:
Please ship at ROOM TEMPERATURE. Please do not use any type of ice.
Template Type | Amount | Concentration | Notes |
---|---|---|---|
Plasmids | 200ng/Primer Extension | >50ng/ul | Submitting extra will allow for re-runs, optimization and troubleshooting. Primers and templates are stored for at least 3 months. We strongly recommend confirming PCR products by gel analysis. |
PCR (>800bp) | 50ng/Primer Extension | >10ng/ul | |
PCR(<800bp) | 10ng/Primer Extension | >2ng/ul |
Amount | Minimum Concentration |
Notes |
---|---|---|
4 pmole/primer extension | 2 uM | Submitting extra will allow for optimization and troubleshooting. Primers and templates are stored for at least 3 months. |
Template Type | Amount of Template | Amount of Primer | Total Volume |
---|---|---|---|
Suspended in water is preferable. | |||
Plasmid | 500 ng | 6 pmoles | 10 µL |
PCR (100bp-500bp) | 10 ng | 6 pmoles | 10 µL |
PCR (500bp-1000bp) | 25 ng | 6 pmoles | 10 µL |
PCR (>1000bp) | 60 ng | 6 pmoles | 10 µL |
Template Type | Service (Click to download form) |
Amount | Concentration |
---|---|---|---|
Plasmids | Single-Strand Coverage | 3 ug per Kb | >0.10ug/ul |
Double-Strand Coverage | 6 ug per Kb | ||
PCR Product | Single-Strand Coverage | 0.3 ug per Kb | >10ng/ul |
Double-Strand Coverage | 0.6 ug per Kb |
Template Type | Instructions/Notes |
---|---|
Single Colonies on Agar Plates (Sequetech Picks the Colonies) | After analyzing the sequences, mini-cultures of the clones of interest can be returned (by request.) |
Single Colonies from Agar Plates (Customer Submits Picked Colonies) | Cells should be picked into 10ul growth media supplemented with the appropriate antibiotic (please identify the antibiotic.) Make sure all tubes and plates are tightly sealed and do not leak. Please do not freeze. |
Liquid Cultures and Glycerol Stocks | We request 10ul of culture grown with the appropriate antibiotic (please identify the antibiotic.) Make sure all tubes and plates are tightly sealed and do not leak. Please do not freeze. |
Partially Purified or Dilute Plasmid Preps | Please supply 10ul of DNA at concentrations as low as 10pg/ul. |
Amount | Minimum Concentration |
Notes |
---|---|---|
4 pmole/primer extension | 2 uM | Submitting extra will allow for optimization and troubleshooting. Primers and templates are stored for at least 3 months. |
Template Requirements (BDX)
Template Type | Amount | Concentration | Notes |
---|---|---|---|
Plasmids * | 200ng/Primer Extension | >50ng/ul | Submitting extra will allow for re-runs, optimization and troubleshooting. Primers and templates are stored for at least 3 months. We strongly recomment confirming PCR products by gel analysis. |
*Turbo PCR Sequencing is recommended for sequencing problematic PCR products.
Amount | Minimum Concentration |
Notes |
---|---|---|
4 pmole/primer extension | 2 uM | Submitting extra will allow for optimization and troubleshooting. Primers and templates are stored for at least 3 months. |
Template Type | Amount | Concentration | Notes |
---|---|---|---|
PCR (>800bp) | 50ng/Primer Extension | >10ng/ul | Submitting extra will allow for optimization and troubleshooting. Primers and templates are stored for at least 3 months. |
PCR(<800bp) | 10ng/Primer Extension | >2ng/ul |
Amount | Minimum Concentration |
Notes |
---|---|---|
4 pmole/primer extension | 2 uM | Submitting extra will allow for optimization and troubleshooting. Primers and templates are stored for at least 3 months. |