Requirements for DNA sequencing
If you are located in the San Francisco Bay Area, please inquire about sample pick-ups.
Please provide a completed Sample Information Sheet along with your samples. Click here to view a list of available forms. We advise that you keep a copy of the completed form for your records.
Suitable starting material for RCA includes circular DNA or host cells containing plasmids to be sequenced.Any circular DNA (including both single and double stranded) can be amplified.
Partially Purified or Dilute Plasmid Preps - Please supply at least 1 µl of DNA at a concentration of at least 10 ng/µl.
Liquid Cultures and Glycerol Stocks - We require at least 50 µl of culture grown with the appropriate antibiotic (please identify antibiotic). To prevent cross contamination, make sure that all tubes and plates are tightly sealed and do not leak. Please do not freeze.
Single Colonies picked from Agar Plates - Cells should be picked into 10 µl of liquid growth media supplemented with the appropriate antibiotic (please identify antibiotic). To prevent cross contamination, make sure that all tubes and plates are tightly sealed and do not leak. Please do not freeze.
Single Colonies on Agar Plates - Sequetech can pick your colonies for you. Upon request, we can return the grown cultures and the original plate. Customers must request this at the time of placing their order, or we will be unable to accommodate the request.
Customers do not need to provide primers for template amplification.The random hexamers included in the RCA reagents can prime synthesis from any circular template.However, customers should provide either gene specific sequencing primers or request the free use of one of our 150+ vector primers.
Linearized plasmids or PCR products are not suitable for this technique.
How to ship samples for RCA