Premix Requirements

Template Type	Amount of Template	Amount of Primer	Total Volume
Suspended in water is preferable.
Plasmid	500 ng	6 pmoles	10 µL
PCR (100bp-500bp)	10 ng	6 pmoles	10 µL
PCR (500bp-1000bp)	25 ng	6 pmoles	10 µL
PCR (>1000bp)	60 ng	6 pmoles	10 µL