Required Primer Amounts and Concentrations
Amount of Primer Required Minimum Concentration
Suspended in 1/10th TE is preferable. Barring that, water is next best.
10µl / Extension 2 µM

Sequetech will provide common vector sequencing primers at no charge. Click here to view a list of vector primers. Customer-provided sequencing primers should be at least 18 bp long, but need not be either gel or HPLC purified. We require that you send at least 15 picomoles of primer per extension at a concentration of at least 3 µM be provided in TE. Excess primer will not be returned unless specifically requested.