*** After over 30 years in business, Sequetech is closing its lab operations permanently and the last orders to be processed must be received by Thurs. 7-20-23. Our billing office remains open and your results will continue to be available for you to access. Thank you for your patronage and support over the years. ***

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Frequently Asked Questions

Q. How far do sequences typically go out?

A: Typically, results for Single Primer Extensions range from 800 to 1000 bases of high-quality data.

Q. What is Sequetech's re-sequencing policy?

A: Sequetech typically re-sequences templates when the data can be improved or if the data was affected by a technical error.

Q. Can Sequetech sequence difficult templates, e.g. G/C rich samples?

A: Yes. Sequetech has several optimized protocols for sequencing difficult templates including denaturing protocols for high G/C templates. Sequetech also encourages their clients to share any information that might be useful in optimizing sequencing requests. Our BDX Chemistry is our most potent weapon against difficult sequences.

Q. Does Sequetech keep my samples? If yes, for how long?

A: Yes. The templates and primers are stored in Sequetech’s freezer archives for up to 6 months from the time of submission.

Q. Can I get samples and/or primers back?

A: Yes. Simply include such instructions in the Sample Information Sheet, which is to be included when submitting your sequencing requests to Sequetech.

Q. Can I put the template and primer in the same tube?

A: Yes. However, we do not recommend this as it limits our ability to optimize difficult reactions. For Sequetech to optimize the sequencing reaction, the primer and template need to be in separate tubes. If you submit template and primer in the same tube, please indicate this on your Sample Information Sheet. For premix requirements, please Click Here.

Q. Can I send my samples in 96-well plates?

A: Yes, absolutely. We recommend standard 96 well plates. The plates can be sealed with strip caps or foil tape.  For detailed shipping information, please Click Here

Q. Can I provide a purchase order (PO) number after I have sent out my samples?

A: No.  We request that you include a PO number or credit card information on your Sample Information Sheet when submitting your sequencing requests to Sequetech.

Q. Does Sequetech synthesize primers?

A: We contract with another company for our Oligonucleotide Synthesis needs. Most primers are available within 24 hours.

Q. Which vector primers do Sequetech provide?

A: Sequetech keeps an extensive catalog of vector primers, which are available for free.  Click here to view a list of our in-house vector primers, or call Sequetech at (800) 697-8685.

Q. How will I receive my results ?

A: Single Primer Extension results can be downloaded as soon as they are available by logging in to your account.  Results are typically available by 10AM the next day.

Q. How much primer do I need to send?

A: Please refer to our Requirements page: Click Here for a detailed table of volume and concentration requirements.

Q. How much template do I need to send?

A: Please refer to our Requirements page: Click Here for a detailed table of volume and concentration requirements.

Q. How should I send my samples?

A: If you are out of the SF Bay Area, we recommend that templates and primers be sent via overnight delivery.  Local customers can request a pickup online or by calling us by 5PM. Make sure that all tubes are sturdy, labeled clearly and are parafilmed closed. Please place all microcentrifuge tubes in 25 or 50 ml screw top tubes.  We recommend using FedEx Priority Service.  For detailed shipping information, please Click Here

Q. What is Sequetech's turnaround time for processing sequencing requests?

A: Although not guaranteed, samples are typically sequenced with data available in less than 24 hours. Samples received by 5:00PM Pacific Time are frequently completed the following day.

Q. What are your hours?

A: Sequetech’s normal business hours are 8AM to 5PM Pacific Time.  If you are a local client, and wish to drop off your samples yourself, you may do so Mondays thru Fridays from 8AM to 11PM Pacific Time. If you are able to bring us the sample by 6:30PM, we will likely be able to process it that evening, so the results will be ready the next day. We also offer a free pick up service if you’d rather not bring the samples to our facility.

Q. Can you receive packages on Saturdays?

A: Sequetech will receive packages on Saturdays only when the client has made special arrangements ahead of time.