A: Typically, results for Single Primer Extensions range from 800 to 1000 bases of high-quality data.
A: Sequetech typically re-sequences templates when the data can be improved or if the data was affected by a technical error.
A: Yes. Sequetech has several optimized protocols for sequencing difficult templates including denaturing protocols for high G/C templates. Sequetech also encourages their clients to share any information that might be useful in optimizing sequencing requests. Our BDX Chemistry is our most potent weapon against difficult sequences.
A: Yes. The templates and primers are stored in Sequetech’s freezer archives for up to 6 months from the time of submission.
A: Yes. Simply include such instructions in the Sample Information Sheet, which is to be included when submitting your sequencing requests to Sequetech.
A: Yes. However, we do not recommend this as it limits our ability to optimize difficult reactions. For Sequetech to optimize the sequencing reaction, the primer and template need to be in separate tubes. If you submit template and primer in the same tube, please indicate this on your Sample Information Sheet. For premix requirements, please Click Here.
A: Yes, absolutely. We recommend standard 96 well plates. The plates can be sealed with strip caps or foil tape. For detailed shipping information, please Click Here
A: No. We request that you include a PO number or credit card information on your Sample Information Sheet when submitting your sequencing requests to Sequetech.
A: We contract with another company for our Oligonucleotide Synthesis needs. Most primers are available within 24 hours.
A: Sequetech keeps an extensive catalog of vector primers, which are available for free. Click here to view a list of our in-house vector primers, or call Sequetech at (800) 697-8685.
A: Single Primer Extension results can be downloaded as soon as they are available by logging in to your account. Results are typically available by 10AM the next day.
A: Please refer to our Requirements page: Click Here for a detailed table of volume and concentration requirements.
A: Please refer to our Requirements page: Click Here for a detailed table of volume and concentration requirements.
A: If you are out of the SF Bay Area, we recommend that templates and primers be sent via overnight delivery. Local customers can request a pickup online or by calling us by 5PM. Make sure that all tubes are sturdy, labeled clearly and are parafilmed closed. Please place all microcentrifuge tubes in 25 or 50 ml screw top tubes. We recommend using FedEx Priority Service. For detailed shipping information, please Click Here
A: Although not guaranteed, samples are typically sequenced with data available in less than 24 hours. Samples received by 5:00PM Pacific Time are frequently completed the following day.
A: Sequetech’s normal business hours are 8AM to 5PM Pacific Time. If you are a local client, and wish to drop off your samples yourself, you may do so Mondays thru Fridays from 8AM to 11PM Pacific Time. If you are able to bring us the sample by 6:30PM, we will likely be able to process it that evening, so the results will be ready the next day. We also offer a free pick up service if you’d rather not bring the samples to our facility.
A: Sequetech will receive packages on Saturdays only when the client has made special arrangements ahead of time.
