Premix Requirements
Template Type Amount of Template Amount of Primer Total Volume
Suspended in water is preferable.
Plasmid 500 ng 6 pmoles 10 µL
PCR (100bp-500bp) 10 ng 6 pmoles 10 µL
PCR (500bp-1000bp) 25 ng 6 pmoles 10 µL
PCR (>1000bp) 60 ng 6 pmoles 10 µL