Frequently Asked Questions
1. How far do sequences typically go out?
2. What is Sequetech's re-sequencing policy?
3. Can Sequetech sequence difficult templates, e.g. G/C rich samples?
4. Does Sequetech keep my samples? If yes, for how long?
5. Can I get samples and/or primers back?
6. Can I put the template and primer in the same tube?
7. Can I send my samples in 96-well plates?
8. Can I provide a purchase order (PO) number after I have sent out my samples?
9. Does Sequetech synthesize primers?
10. Which vector primers do Sequetech provide?
11. How will I receive my results?
12. How much primer do I need to send?
13. How much template do I need to send?
14. How should I send my samples?
15. What is Sequetech's turnaround time for processing sequencing requests?
16. What are your hours?
17. Can you receive packages on Saturdays?
Q. How far do sequences typically go out?
A: Typically, results for Single Primer Extensions range from 800 to 1000 bases of high-quality data.
Q. What is Sequetech's re-sequencing policy?
A: Sequetech typically re-sequences templates when the data can be improved or if the data was affected by a technical error.
Q. Can Sequetech sequence difficult templates, e.g. G/C rich samples?
A: Yes. Sequetech has several optimized protocols for sequencing difficult templates including denaturing protocols for high G/C templates. Sequetech also encourages their clients to share any information that might be useful in optimizing sequencing requests.
Q. Does Sequetech keep my samples? If yes, for how long?
A: Yes. The templates and primers are stored in Sequetech's freezer archives for up to 6 months from the time of submission.
Q. Can I get samples and/or primers back?
A: Yes. Simply include such instructions in the Sample Information Sheet, which is to be included when submitting your sequencing requests to Sequetech.
Q. Can I put the template and primer in the same tube?
A: Yes. However, for Sequetech to optimize the sequencing reaction, the primer and template need to be in separate tubes. If you submit template and primer in the same tube, please indicate this on your Sample Information Sheet. For premix requirements, please Click Here.
Q. Can I send my samples in 96-well plates?
A: Yes, absolutely. We recommend standard semi skirted 96 well plates that will fit into a thermocycler. The plates can be sealed with strip caps or foil tape. It is strongly recommended that plates be shipped frozen, on dry ice, to avoid leakage issues and well to well contamination. If using strip caps, we suggest wrapping the plate in parafilm to further secure the strip caps in position. For detailed shipping information, please Click Here
Q. Can I provide a purchase order (PO) number after I have sent out my samples?
A: No. We request that you include a PO number or credit card information on your Sample Information Sheet when submitting your sequencing requests to Sequetech.
Q. Does Sequetech synthesize primers?
A: We contract with another company for our Oligonucleotide Synthesis needs. Most primers are available within 24 hours.
Q. Which vector primers do Sequetech provide?
A: Sequetech keeps an extensive catalog of vector primers, which are available for free. Click here to view a list of our in-house vector primers, or call Sequetech at (800) 697-8685.
Q. How will I receive my results ?
A: We generally send results via eMail. If requested, we will also ship (via US mail, or FedEx) the 4-color prints of the chromatograms, as well as the sequencing results in either PC or MAC file format. You may specify your preferred format in the Sample Information Sheet, when submitting your requests.
Q. How should I send my samples?
A: We recommend that templates and primers be sent via overnight delivery on dry ice or with frozen cooling blocks. Make sure that all tubes are sturdy, labeled clearly and are parafilmed closed. Please place all microcentrifuge tubes in 25 or 50 ml screw top tubes. We recommend using FedEx Priority Service. For detailed shipping information, please Click Here
Q. What is Sequetech's turnaround time for processing sequencing requests?
A: Although not guaranteed, samples are typically sequenced with data available in less than 24 hours. Samples received by 5:00PM Pacific Time are frequently completed the following day.
Q. What are your hours?
A: Sequetech's normal business hours are 8AM to 5PM Pacific Time. If you are a local client, and wish to drop off your samples yourself, you may do so Mondays thru Fridays from 8AM to 11PM Pacific Time. If you are able to bring us the sample by 6:30PM, we will likely be able to process it that evening, so the results will be ready the next day. We also offer a free pick up service if you'd rather not bring the samples to our facility.
Q. Can you receive packages on Saturdays?
A: Sequetech will receive packages on Saturdays only when the client has made special arrangements ahead of time.